Title
FISH analysis of **Lactobacillus** biofilms in the gastrointestinal tract of different hosts FISH analysis of **Lactobacillus** biofilms in the gastrointestinal tract of different hosts
Author
Faculty/Department
Faculty of Sciences. Bioscience Engineering
Publication type
article
Publication
Oxford ,
Subject
Biology
Human medicine
Engineering sciences. Technology
Source (journal)
Letters in applied microbiology. - Oxford
Volume/pages
52(2011) :3 , p. 220-226
ISSN
0266-8254
ISI
000287038400006
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Aims: To investigate the spatial organization of endogenous and exogenously applied Lactobacillus communities at specific locations in the adult gastrointestinal tract of different hosts. Methods and Results: Samples of the human, murine and avian gastrointestinal tract of subjects that received or not received a Lactobacillus probiotic were analysed by fluorescence in situ hybridization (FISH) with rRNA-targeted probes. High levels of endogenous lactobacilli were observed on the nonsecretory, stratified squamous epithelia present in the forestomach of mice and crop of chickens, respectively. These epithelial associations showed characteristics of bacterial biofilms, i.e. bacteria attached to a surface and embedded in a matrix of extracellular polymeric substances. In other regions of the analysed intestines, lactobacilli seemed to occur mainly as dispersed bacterial cells or as microcolonies. Exogenous administration of a Lactobacillus probiotic did increase the levels of loosely adherent Lactobacillus cells detected. However, the probiotic strains were unable to establish themselves inside the gastrointestinal biofilms. Conclusions: Gastrointestinal biofilms of lactobacilli occur only in specific niches in certain hosts, such as the murine forestomach and avian crop. Significance and Impact of the Study: Biofilm formation by lactobacilli in specific parts of animal gastrointestinal tracts was documented for the first time by FISH.
E-info
https://repository.uantwerpen.be/docman/iruaauth/17cba0/cb7bf803e8c.pdf
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