Publication
Title
Dried blood spot punches for confirmation of suspected -hydroxybutyric acid intoxications : validation of an optimized GCMS procedure
Author
Abstract
Background: γ-hydroxybutyric acid (GHB), notorious as a club- and date-rape drug, was quantified in dried blood spots (DBS) by punching out a disc, followed by on-spot derivatization and analysis by GCMS. Results: A homogenous distribution in DBS was demonstrated and accurate results were obtained when analyzing a disc punched out from a 2035 µl spot, regardless the hematocrit of the blood sample. Validation based on US FDA and European Medicines Agency guidelines was performed, with a calibration range covering 2100 µg/ml. Conclusion: A sensitive GCMS method for GHB analysis in DBS was successfully optimized and validated. The successful analysis of DBS collected from GHB abusers suggests the routine applicability of the DBS sampling technique for GHB analysis in toxicological cases.
Language
English
Source (journal)
Bioanalysis
Publication
2011
ISSN
17576180
Volume/pages
3:20(2011), p. 2271-2281
ISI
000296921000009
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 26.01.2012
Last edited 20.09.2017
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