Title
Dried blood spot punches for confirmation of suspected <tex>$\gamma$</tex>-hydroxybutyric acid intoxications : validation of an optimized GCMS procedure Dried blood spot punches for confirmation of suspected <tex>$\gamma$</tex>-hydroxybutyric acid intoxications : validation of an optimized GCMS procedure
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
Subject
Chemistry
Biology
Pharmacology. Therapy
Human medicine
Source (journal)
Bioanalysis
Volume/pages
3(2011) :20 , p. 2271-2281
ISSN
1757-6180
ISI
000296921000009
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Background: γ-hydroxybutyric acid (GHB), notorious as a club- and date-rape drug, was quantified in dried blood spots (DBS) by punching out a disc, followed by on-spot derivatization and analysis by GCMS. Results: A homogenous distribution in DBS was demonstrated and accurate results were obtained when analyzing a disc punched out from a 2035 µl spot, regardless the hematocrit of the blood sample. Validation based on US FDA and European Medicines Agency guidelines was performed, with a calibration range covering 2100 µg/ml. Conclusion: A sensitive GCMS method for GHB analysis in DBS was successfully optimized and validated. The successful analysis of DBS collected from GHB abusers suggests the routine applicability of the DBS sampling technique for GHB analysis in toxicological cases.
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