Title
Mixture designs in the optimisation of PLGA nanoparticles : influence of organic phase composition on <tex>$\beta$</tex>-aescin encapsulation Mixture designs in the optimisation of PLGA nanoparticles : influence of organic phase composition on <tex>$\beta$</tex>-aescin encapsulation
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Publication type
article
Publication
London ,
Subject
Chemistry
Pharmacology. Therapy
Source (journal)
Journal of microencapsulation. - London
Volume/pages
29(2012) :2 , p. 115-125
ISSN
0265-2048
ISI
000300261600002
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The objective of this study was to enhance the encapsulation of the antileishmanial saponin aescin in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). We prepared the NPs by the O/W and W/O/W combined emulsification solvent evaporation/salting-out technique and investigated the influence of organic phase composition on the NPs size, zeta potential and entrapment efficiency (EE%) using mixture designs. The obtained NPs were monodispersed with Zave<300 nm and exhibited negative zeta potentials. For the single emulsion, the co-solvent concentration was shown to be the primary determinant of drug entrapment. The EE% increased from 14% to 22% by decreasing the amount of DMSO from 80% to 25% (v/v) in the organic polymer solution. For the double emulsion, EE% was 22% on average and independent of the organic phase composition. The double-emulsion technique did not enhance the aescin encapsulation as expected due to its amphiphilic nature. The optimised aescin-loaded NPs meet the requirements for further in vitro activity tests.
E-info
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