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Title
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Mixture designs in the optimisation of PLGA nanoparticles : influence of organic phase composition on -aescin encapsulation
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Author
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Abstract
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| The objective of this study was to enhance the encapsulation of the antileishmanial saponin aescin in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). We prepared the NPs by the O/W and W/O/W combined emulsification solvent evaporation/salting-out technique and investigated the influence of organic phase composition on the NPs size, zeta potential and entrapment efficiency (EE%) using mixture designs. The obtained NPs were monodispersed with Zave<300 nm and exhibited negative zeta potentials. For the single emulsion, the co-solvent concentration was shown to be the primary determinant of drug entrapment. The EE% increased from 14% to 22% by decreasing the amount of DMSO from 80% to 25% (v/v) in the organic polymer solution. For the double emulsion, EE% was 22% on average and independent of the organic phase composition. The double-emulsion technique did not enhance the aescin encapsulation as expected due to its amphiphilic nature. The optimised aescin-loaded NPs meet the requirements for further in vitro activity tests. |
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Language
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English
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Source (journal)
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Journal of microencapsulation. - London
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Publication
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London
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2012
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ISSN
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0265-2048
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DOI
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10.3109/02652048.2011.630108
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Volume/pages
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29
:2
(2012)
, p. 115-125
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ISI
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000300261600002
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Full text (Publisher's DOI)
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