Publication
Title
Mixture designs in the optimisation of PLGA nanoparticles : influence of organic phase composition on $\beta$-aescin encapsulation
Author
Abstract
 The objective of this study was to enhance the encapsulation of the antileishmanial saponin aescin in poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs). We prepared the NPs by the O/W and W/O/W combined emulsification solvent evaporation/salting-out technique and investigated the influence of organic phase composition on the NPs size, zeta potential and entrapment efficiency (EE%) using mixture designs. The obtained NPs were monodispersed with Zave<300 nm and exhibited negative zeta potentials. For the single emulsion, the co-solvent concentration was shown to be the primary determinant of drug entrapment. The EE% increased from 14% to 22% by decreasing the amount of DMSO from 80% to 25% (v/v) in the organic polymer solution. For the double emulsion, EE% was 22% on average and independent of the organic phase composition. The double-emulsion technique did not enhance the aescin encapsulation as expected due to its amphiphilic nature. The optimised aescin-loaded NPs meet the requirements for further in vitro activity tests.
Language
English
Source (journal)
Journal of microencapsulation. - London
Journal of microencapsulation. - London
Publication
London : 2012
ISSN
0265-2048
Volume/pages
29 :2 (2012) , p. 115-125
ISI
000300261600002
Full text (Publisher's DOI)
UAntwerpen
 Faculty/Department Research group Publication type Subject Affiliation Publications with a UAntwerp address