Title
In situ localisation of cytokinins in the shoot apical meristem of **Sinapis alba** at floral transition In situ localisation of cytokinins in the shoot apical meristem of **Sinapis alba** at floral transition
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Berlin ,
Subject
Biology
Source (journal)
Planta. - Berlin
Volume/pages
214(2002) :6 , p. 970-973
ISSN
0032-0935
ISI
000175309400019
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
In plants of Sinapis alba L. induced to flower by one long day (LD), previous work showed that the phloem sap feeding the shoot apex is enriched in cytokinins of the isopentenyladenine (iP)-type between 9 and 25 h after start of the LID [P. Lejeune et al. (1994) Physiol Plant 90:522-528]. We have checked the hypothesis that the cytokinin content of the shoot apical meristem (SAM) should increase in response to floral induction by one LD using histoimmunolocalisation techniques and rabbit antiserum against isopentenyladenosine or zeatin riboside. The free bases iP and zeatin are present only in apical tissues containing dividing cells. At 30 h after the start of an inductive LID, a markedly increased iP immune reaction is observed in SAM tissues while the level of zeatin is not modified. Our results are in line with the data obtained by analysis of phloem sap.
E-info
https://repository.uantwerpen.be/docman/iruaauth/3d6e07/9322081.pdf
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