Publication
Title
Immunological assay for the determination of procarboxypeptidase U antigen levels in human plasma
Author
Abstract
The importance of carboxypeptidase U as a novel regulator of the fibrinolytic rate has attracted a lot of interest recently, in the present work, an ELISA was developed using polyclonal antibodies raised against recombinant proCPU, expressed in DON cells. The assay determines the antigen concentration of the zymogen of carboxypeptidase U, procarboxypeptidase U, in human citrated plasma or EDTA plasma. No interference is observed with plasma carboxypeptidase N. The assay is very reproducible (within-run: 4.6% CV, between-run: 6.8% CV). In a group of 479 healthy individuals the mean proCPU antigen concentration is 13.4 mug/ml (SD 2.5 mug/ml). A good correlation is found with the functional procarboxypeptidase U assay described earlier (r = 0.82, p <0.0001) (Schatteman K, Goossens F, Scharpe S, Neels H, Hendriks D Clin Chem 1999; 45: 807-813). The significant correlation between the proCPU antigen concentration and the 50% clot lysis time stresses its importance as a player in fibrinolysis control.
Language
English
Source (journal)
Thrombosis and haemostasis. - Stuttgart, 1987
Publication
Stuttgart : Schattauer, 2001
ISSN
0340-6245
Volume/pages
85:1(2001), p. 12-17
ISI
000166544100004
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 24.02.2012
Last edited 21.06.2017
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