Title
Immunological assay for the determination of procarboxypeptidase U antigen levels in human plasmaImmunological assay for the determination of procarboxypeptidase U antigen levels in human plasma
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Research group
Department of Pharmaceutical Sciences - other
Medical Biochemistry
Department of Pharmaceutical Sciences
Publication type
article
Publication
Stuttgart :Schattauer,
Subject
Human medicine
Source (journal)
Thrombosis and haemostasis. - Stuttgart, 1987
Volume/pages
85(2001):1, p. 12-17
ISSN
0340-6245
ISI
000166544100004
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
The importance of carboxypeptidase U as a novel regulator of the fibrinolytic rate has attracted a lot of interest recently, in the present work, an ELISA was developed using polyclonal antibodies raised against recombinant proCPU, expressed in DON cells. The assay determines the antigen concentration of the zymogen of carboxypeptidase U, procarboxypeptidase U, in human citrated plasma or EDTA plasma. No interference is observed with plasma carboxypeptidase N. The assay is very reproducible (within-run: 4.6% CV, between-run: 6.8% CV). In a group of 479 healthy individuals the mean proCPU antigen concentration is 13.4 mug/ml (SD 2.5 mug/ml). A good correlation is found with the functional procarboxypeptidase U assay described earlier (r = 0.82, p <0.0001) (Schatteman K, Goossens F, Scharpe S, Neels H, Hendriks D Clin Chem 1999; 45: 807-813). The significant correlation between the proCPU antigen concentration and the 50% clot lysis time stresses its importance as a player in fibrinolysis control.
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