Title
Oestrogenic activity of CPRG (chlorophenol red-beta-D-galactopyranoside), a beta-galactosidase substrate commonly used in recombinant yeast oestrogenic assays Oestrogenic activity of CPRG (chlorophenol red-beta-D-galactopyranoside), a beta-galactosidase substrate commonly used in recombinant yeast oestrogenic assays
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Subject
Biology
Pharmacology. Therapy
Engineering sciences. Technology
Source (journal)
Biomarkers: biochemical indicators of exposure, response, and susceptibility to chemicals
Volume/pages
6(2001) :5 , p. 375-380
ISSN
1354-750X
ISI
000170235200007
Carrier
E
Target language
English (eng)
Affiliation
University of Antwerp
Abstract
The finding that a variety of chemicals display oestrogenic activity has resulted in the development of in vitro and in vivo assays to assess oestrogenic activity. One such assay, the yeast oestrogen assay (YES) makes use of recombinant yeast cells that harbour an oestrogen receptor expression cassette and a reporter construct, coding for beta -galactosidase. The induction mechanism starts with the binding of oestrogenic compounds to the oestrogen receptor. This complex activates the production of beta -galactosidase. The beta -galactosidase activity is thus a measure of the oestrogenic activity of chemical compounds. In the YES assay, the beta -galactosidase activity may be quantified with the chromogenic substrate chlorophenol red-beta -D-galactopyranoside (CPRG). In the present study it is reported that CPRG or its beta -galactosidase degradation product chlorophenol red act in the YES as an oestrogenic compound itself. The implications of this finding are described. It is especially argued that chlorophenol red production after prolonged incubation of the assay might be misinterpreted as an oestrogenic effect of the test compound.
E-info
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