Publication
Title
Nimodipine and flunarizine have different effects on survival and morphology of PC12 cells during nerve growth factor deprivation
Author
Abstract
The purpose of this study was to examine the effect of antagonists of different subtypes of Ca2+ channels (nimodipine and flunarizine) and two types of Ca2+ chelating agents (the cell permeant Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethylester (BAPTA-AM) and the cell non-permeant Ca2+ chelator EGTA) on neurite retraction and cell death of nerve growth factor (NGF)-differentiated PC12 cells after NGF deprivation. We demonstrated that flunarizine and nimodipine, but not BAPTA-AM and EGTA, provided protection against cell death due to NGF deprivation. Using time-lapse videomicroscopy and quantitative image analysis, we found that retraction of neurites was an early and fast phenomenon after removal of NGF. None of the compounds tested (flunarizine, nimodipine, BAPTA-AM, EGTA) could prevent the retraction of neurites. (C) 1999 Elsevier Science B.V. All rights reserved.
Language
English
Source (journal)
European journal of pharmacology. - Amsterdam
Publication
Amsterdam : 1999
ISSN
0014-2999
Volume/pages
384:1(1999), p. 61-70
ISI
000083960500010
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 29.02.2012
Last edited 16.06.2017
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