Title
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Nimodipine and flunarizine have different effects on survival and morphology of PC12 cells during nerve growth factor deprivation
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Author
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Abstract
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The purpose of this study was to examine the effect of antagonists of different subtypes of Ca2+ channels (nimodipine and flunarizine) and two types of Ca2+ chelating agents (the cell permeant Ca2+ chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid acetoxymethylester (BAPTA-AM) and the cell non-permeant Ca2+ chelator EGTA) on neurite retraction and cell death of nerve growth factor (NGF)-differentiated PC12 cells after NGF deprivation. We demonstrated that flunarizine and nimodipine, but not BAPTA-AM and EGTA, provided protection against cell death due to NGF deprivation. Using time-lapse videomicroscopy and quantitative image analysis, we found that retraction of neurites was an early and fast phenomenon after removal of NGF. None of the compounds tested (flunarizine, nimodipine, BAPTA-AM, EGTA) could prevent the retraction of neurites. (C) 1999 Elsevier Science B.V. All rights reserved. |
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Language
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English
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Source (journal)
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European journal of pharmacology. - Amsterdam, 1967, currens
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Publication
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Amsterdam
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North-Holland
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1999
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ISSN
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0014-2999
[print]
1879-0712
[online]
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DOI
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10.1016/S0014-2999(99)00665-2
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Volume/pages
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384
:1
(1999)
, p. 61-70
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ISI
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000083960500010
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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