Title
Evaluation of the swimming activity of Daphnia magna by image analysis after administration of sublethal Cadmium concentrations Evaluation of the swimming activity of Daphnia magna by image analysis after administration of sublethal Cadmium concentrations
Author
Faculty/Department
Faculty of Sciences. Biology
Faculty of Sciences. Physics
Publication type
article
Publication
London ,
Subject
Chemistry
Biology
Human medicine
Source (journal)
Comparative biochemistry and physiology : A : molecular & integrative physiology. - London
Volume/pages
120(1998) :1 , p. 99-105
ISSN
1095-6433
ISI
000075437300016
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The swimming activity of Daphnia magna was determined by a real time image analysis, using a video camera and a PC equipped with a standard low cost frame grabber. For a sequence of 20 images, where 30 organisms are moving simultaneously, the trajectories have been reconstructed in a binary image. The average velocity was derived from the statistical analysis of these trajectories, the participation in swimming activity and the distribution (or migration) of the organisms in the measuring cell are derived from data analysis. The swimming activity of the organisms has been measured in normal conditions and after application of an acute sublethal cadmium stress (concentrations of 3.5 and 5.0 mu g l(-1)). This metal stress induced an important decrease of the swimming activity. Application of a rise of temperature (from 20 to 25 degrees C) in combination with the sublethal cadmium stress amplified this decrease. The alterations of the swimming velocity, participation and distribution have been quantified. The results were available within 24 h. (C) 1998 Elsevier Science Inc. All rights reserved.
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