Publication
Title
Simultaneous purification of the neuroproteins synapsin-i and synaptophysin
Author
Abstract
A procedure for the simultaneous purification of synapsin I and synaptophysin from calf brain was developed. Demyelinated membranes were extracted with 2% Triton X-100 and 2 M KCl. The extracted proteins were separated by weak cation-exchange chromatography on carboxymethyl-Sepharose Fast Flow. Synaptophysin was finally purified by preparative sodium dodecyl sulphate-polyacrylamine gel electrophoresis and synapsin I by affinity chromatography using a calmodulin-Sepharose column. The recovery obtained was 40-mu-g/g in brain for synaptophysin and 25-mu-g/g in brain for synapsin I.
Language
English
Source (journal)
Journal of chromatography: A. - Amsterdam
Publication
Amsterdam : 1992
ISSN
0021-9673
Volume/pages
596:1(1992), p. 51-58
ISI
A1992HP08800005
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 29.02.2012
Last edited 17.11.2017