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Title
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Simultaneous purification of the neuroproteins synapsin-i and synaptophysin
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Author
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Abstract
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| A procedure for the simultaneous purification of synapsin I and synaptophysin from calf brain was developed. Demyelinated membranes were extracted with 2% Triton X-100 and 2 M KCl. The extracted proteins were separated by weak cation-exchange chromatography on carboxymethyl-Sepharose Fast Flow. Synaptophysin was finally purified by preparative sodium dodecyl sulphate-polyacrylamine gel electrophoresis and synapsin I by affinity chromatography using a calmodulin-Sepharose column. The recovery obtained was 40-mu-g/g in brain for synaptophysin and 25-mu-g/g in brain for synapsin I. |
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Language
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English
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Source (journal)
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Journal of chromatography : A. - Amsterdam, 1958, currens
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Publication
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Amsterdam
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Elsevier
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1992
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ISSN
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0021-9673
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DOI
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10.1016/0021-9673(92)80201-5
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Volume/pages
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596
:1
(1992)
, p. 51-58
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ISI
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A1992HP08800005
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Full text (Publisher's DOI)
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