Title
Simultaneous purification of the neuroproteins synapsin-i and synaptophysin Simultaneous purification of the neuroproteins synapsin-i and synaptophysin
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Amsterdam ,
Subject
Chemistry
Source (journal)
Journal of chromatography: A. - Amsterdam
Volume/pages
596(1992) :1 , p. 51-58
ISSN
0021-9673
ISI
A1992HP08800005
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
A procedure for the simultaneous purification of synapsin I and synaptophysin from calf brain was developed. Demyelinated membranes were extracted with 2% Triton X-100 and 2 M KCl. The extracted proteins were separated by weak cation-exchange chromatography on carboxymethyl-Sepharose Fast Flow. Synaptophysin was finally purified by preparative sodium dodecyl sulphate-polyacrylamine gel electrophoresis and synapsin I by affinity chromatography using a calmodulin-Sepharose column. The recovery obtained was 40-mu-g/g in brain for synaptophysin and 25-mu-g/g in brain for synapsin I.
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