Lack of galectin-3 alleviates trypanosomiasis-associated anemia of inflammationLack of galectin-3 alleviates trypanosomiasis-associated anemia of inflammation
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Veterinary Sciences
Applied veterinary morphology
Immunobiology. - Stuttgart, 1979, currens
215(2010):9-10, p. 833-841
University of Antwerp
A typical pathological feature associated with experimental African trypanosomiasis (Trypanosoma brucei infection in mice) is anemia of chronic disease (ACD), which is due to a sustained type 1 cytokine-mediated inflammation and hyperactivation of M1 macrophages. Galectin-3 (Gal-3) was amply documented to contribute to the onset and persistence of type 1 inflammatory responses and we herein document that this protein is strongly upregulated during T. brucei infection. We evaluated the involvement of Gal-3 in trypanosomiasis-associated anemia using galectin-3 deficient (Ga13(-/-)) mice. T. brucei infected Ga13-/- mice manifested significant lower levels of anemia during infection and survived twice as long as wild type mice. Moreover, such mice showed increased levels of serum IL-10 and reduced liver pathology (as evidenced by lower AST/ALT levels). In addition, there was also an increase in gene expression of iron export genes and a reduced expression of genes, which are associated with accumulation of cellular iron. Our data indicate that Gal-3 is involved in the development of inflammation-associated anemia during African trypanosomiasis, possibly due to a disturbed iron metabolism that in turn may also lead to liver malfunction. (C) 2010 Elsevier GmbH. All rights reserved.