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Title
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Cytomegalovirus DNA detection in Guthrie cards : role in the diagnostic work-up of childhood hearing loss
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Author
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Abstract
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| Introduction: Cytomegalovirus (CMV) infection is the leading cause of congenital nongenetic sensorineural hearing loss (SNHL) and a major cause of prelingual SNHL that is not present at birth. Polymerase chain reaction (PCR) analysis of dried blood samples on the Guthrie card has been proposed as a sensitive and specific method to screen for congenital CMV infection. Methods: Prospectively, consecutive infants who failed universal neonatal hearing screening and children referred for a non-congenital SNHL (NCHL) were included and underwent a standard audiometric and etiologic work-up. DNA was extracted from dried blood spots on neonatal Guthrie cards and amplified by real-time PCR. Data were available for 96 cases. Results: Mean age of the universal neonatal hearing screening group was 3.8 +/- 2.4 months (n = 41). Auditory brain stem response thresholds were 72.9 +/- 20.2 dB nHL. A CMV-positive PCR was obtained in 4 babies. One test was considered false-positive. This resulted in a 7.3% prevalence of congenital CMV infections. Mean age of the NCHL group was 4.9 +/- 3.2 years (n = 55). Hearing loss was moderate in 37, severe in 5, and profound in 13 children. A CMV-positive PCR was obtained in 4 children (7.3%). Other causes of SNHL were excluded in the PCR positive cases of both study groups. Conclusion: We advocate PCR for CMV DNA detection on Guthrie cards in the etiologic work-up of childhood SNHL and recommend serologic confirmation to exclude false-positive PCR results. 7.3% of SNHL in babies with congenital hearing loss and children with NCHL could be attributed with this technique to congenital CMV infection. |
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Language
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English
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Source (journal)
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Otology and neurotology. - Philadelphia, Pa.
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Publication
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Philadelphia, Pa.
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2009
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ISSN
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1531-7129
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DOI
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10.1097/MAO.0B013E3181B76B22
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Volume/pages
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30
:7
(2009)
, p. 943-949
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ISI
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000276926500013
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Full text (Publisher's DOI)
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