Title
|
|
|
|
Changes in the expression of -adrenergic receptor subtypes during maturation of neuronal cells from fetal pig superior cervical ganglia
|
|
Author
|
|
|
|
|
|
Abstract
|
|
|
|
The expression of presynaptic alpha(2)-adrenergic receptor (alpha(2)-AR) subtypes was investigated in cultured neurons from fetal pig superior cervical ganglion (SCG). Cells were incubated with chicken antibodies against alpha(2)A-, alpha(2)B- or alpha(2)C-AR subtypes either alone or together with antibodies against dopamine-beta-hydroxylase (D beta H, a marker for adrenergic neurons) or against choline acetyl transferase (ChAT, a marker for cholinergic neurons). We found immunoreactivity for ail three alpha(2)-AR subtypes in SCG-cells when cultured for 8-11 days. The relative expression of the alpha(2)A-subtype was approximately 1/3 of that of alpha(2)B- and alpha(2)C-AR. Co-localisation of all three alpha(2)-AR subtypes was observed in cells expressing DPH or ChAT. Increasing the potassium concentration in the culture medium increased the expression of DPH and decreased the expression of the alpha(2)A- and alpha(2)C-subtype without altering the expression of the alpha(2)B-subtype. Co-culture of neurons with pig splenocytes enhanced the expression of ChAT and decreased the expression of the alpha(2)B-subtype without altering the expression of alpha(2)A- and alpha(2)C-subtypes. Our results indicate that the three alpha(2)-receptor subtypes are expressed on both noradrenergic and cholinergic nerves. Induction of the noradrenergic phenotype favours the expression of the alpha(2)B-subtype over that of the alpha(2)A- and alpha(2)C-subtype. Conversely, enhancement of the cholinergic phenotype favours the expression of the alpha(2)A- and alpha(2)C-subtypes over that of the alpha(2)B-subtype. Our results suggest that the alpha(2)B-receptor is preferentially associated with noradrenergic nerve endings. (C) 1999 Elsevier Science B.V. All rights reserved. |
|
|
Language
|
|
|
|
English
|
|
Source (journal)
|
|
|
|
Developmental brain research. - Amsterdam
|
|
Publication
|
|
|
|
Amsterdam
:
1999
|
|
ISSN
|
|
|
|
0165-3806
|
|
DOI
|
|
|
|
10.1016/S0165-3806(99)00078-4
|
|
Volume/pages
|
|
|
|
116
:2
(1999)
, p. 127-132
|
|
ISI
|
|
|
|
000082667800001
|
|
Full text (Publisher's DOI)
|
|
|
|
|
|