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Title
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Flow cytometric analysis of erythrocytic D antigen density profile
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Author
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Abstract
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| Red blood cells (RBC) were studied with flow cytometry (FCM) after staining with a directly fluoresceinated anti-D antiserum. The immunofluorescence distribution profiles were slightly asymmetrical, due to a significant, yet small skewness. This skewness was not associated specifically with D antigen positivity, making a homogeneous, normal D antigen distribution on the RBC very likely. With the exception of Du, D-positive RBC could always be distinguished from D-negative ones. FCM was successful in bringing the following phenomena to light: difference in mean fluorescence intensity between the different rhesus phenotypes, the D gene dosage effect, the depressing effect of C antigen on D antigenicity, the nonspecific attachment of IgG on (D-negative) RBC and rhesus mosaicism. The absolute number of D antigen sites could be calculated, using an absolute fluorescence standard. |
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Language
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English
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Source (journal)
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Vox sanguinis. - Basel
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Publication
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Basel
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1986
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ISSN
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0042-9007
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DOI
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10.1111/J.1423-0410.1986.TB00206.X
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Volume/pages
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51
:1
(1986)
, p. 40-46
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ISI
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A1986D348100006
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Full text (Publisher's DOI)
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