Title
A multicenter evaluation of the routine use of a new white cell- reduction apheresis system for collection of platelets A multicenter evaluation of the routine use of a new white cell- reduction apheresis system for collection of platelets
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Arlington, Va ,
Subject
Human medicine
Source (journal)
Transfusion / AABB. - Arlington, Va, 1961, currens
Volume/pages
37(1997) :5 , p. 487-492
ISSN
0041-1132
1537-2995
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
BACKGROUND: Residual white cells (WBCs) cause serious side effects in platelet transfusion. An in-line WBC-reduction system based on fluidized particle bed technology was recently developed as a modification of an existing plateletpheresis system. STUDY DESIGN AND METHODS: In an investigational phase, three flow profiles were evaluated using prototype software in five centers, each using their standard conditions. In the confirmatory phase, the released software was tested in three centers. WBCs were counted in two full Nageotte grids (dilution 1-in-5). RESULTS: With the prototype software, WBC levels were always below 1 × 106 per procedure (median, 25,000/procedure; n = 314). One profile proved to be superior to the other two with respect to platelet yield and residual WBCs, and it was incorporated in the released WBC-reduction system, together with a built-in process control. Median residual WBCs in these WBC-reduction system components not rejected by the process control were 19,000 per procedure (n = 211/225 total), with 99.5 percent of the platelet components having less than 1 × 106 WBCs. CONCLUSION: The protocol selected in the initial phase, now available as a WBC-reduction system, results in platelet concentrates with very low residual WBC levels. This satisfies even the most stringent criteria for WBC reduction in platelets, without the platelet loss typically seen with conventional fiber filtration.
E-info
https://repository.uantwerpen.be/docman/iruaauth/a697b9/73d193e66a8.pdf
Handle