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Title
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Feasibility study of the zebrafish assay as an alternative method to screen for developmental toxicity and embryotoxicity using a training set of 27 compounds
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Author
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Abstract
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| To anticipate to increased testing needs for reproductive toxicity and 3R approaches, we studied zebrafish embryo/larva as an alternative for animal testing for developmental toxicity and embryotoxicity and evaluated a training set of 27 compounds with a standardized protocol. The classification of compounds in the zebrafish embryo/larva assay, based on a prediction model using a TI (teratogenic index) cut-off value of 2, was compared to available animal and human data. When comparing the classification of compounds in the zebrafish embryo/larva assay to available animal classification, a sensitivity of 72% and specificity of 100% were obtained. The predictive values obtained in comparison to a limited set of human data were 50, 60% respectively for teratogens, non-teratogens. Overall, we demonstrated that the zebrafish embryo/larva assay, may be used as screening tool for prioritization of compounds and could contribute to reduction of animal experiments in the field of developmental toxicology. |
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Language
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English
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Source (journal)
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Reproductive toxicology. - Elmsford, N.Y.
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Publication
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Elmsford, N.Y.
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2012
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ISSN
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0890-6238
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DOI
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10.1016/J.REPROTOX.2011.08.003
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Volume/pages
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33
:2
(2012)
, p. 142-154
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ISI
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000302204700004
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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