Title
Feasibility study of the zebrafish assay as an alternative method to screen for developmental toxicity and embryotoxicity using a training set of 27 compounds
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Elmsford, N.Y. ,
Subject
Biology
Pharmacology. Therapy
Source (journal)
Reproductive toxicology. - Elmsford, N.Y.
Volume/pages
33(2012) :2 , p. 142-154
ISSN
0890-6238
ISI
000302204700004
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
To anticipate to increased testing needs for reproductive toxicity and 3R approaches, we studied zebrafish embryo/larva as an alternative for animal testing for developmental toxicity and embryotoxicity and evaluated a training set of 27 compounds with a standardized protocol. The classification of compounds in the zebrafish embryo/larva assay, based on a prediction model using a TI (teratogenic index) cut-off value of 2, was compared to available animal and human data. When comparing the classification of compounds in the zebrafish embryo/larva assay to available animal classification, a sensitivity of 72% and specificity of 100% were obtained. The predictive values obtained in comparison to a limited set of human data were 50, 60% respectively for teratogens, non-teratogens. Overall, we demonstrated that the zebrafish embryo/larva assay, may be used as screening tool for prioritization of compounds and could contribute to reduction of animal experiments in the field of developmental toxicology.
E-info
https://repository.uantwerpen.be/docman/iruaauth/7a3525/0521a924191.pdf
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