Publication
Title
Detection of hypoxia inducible factors and angiogenic growth factors during foetal endochondral and intramembranous ossification
Author
Abstract
During skeletogenesis, the development of a new vascular network, i.e. angiogenesis, is triggered by hypoxia through the activation of the hypoxia inducible factors (HIFs) HIF-1α and HIF-2α. HIFs regulate the expression of several genes, including those coding for angiogenic growth factors such as VEGFA, angiopoietin-1 (ANGPT1) and angiopoietin-2 (ANGPT2). The expression of HIFs and angiogenic growth factors is well documented in endochondral ossification, but few data exist on their expression during intramembranous ossification. In this study, the localization of these factors was examined using immunohistochemistry and RT-PCR in bones of porcine foetuses. Immunostaining for HIF-1α and HIF-2α was observed during endochondral ossification, whereas only HIF-2α was present at sites of intramembranous ossification. Furthermore, immunostaining for ANGPT2 was present at sites of endochondral and intramembranous ossification. In addition, gene transcripts for ANGPT1, ANGPT2 and VEGFA were detected with RT-PCR in laser capture microdissected isolates from both types of ossification. These results indicate that angiogenesis plays an important role during endochondral and intramembranous ossification. However, the different expression pattern of the HIF-α subunits suggests that alternative regulatory pathways trigger angiogenesis in these distinct types of ossification.
Language
English
Source (journal)
Anatomia, histologia, embryologia: journal of veterinary medicine: series C. - Berlin
Publication
Berlin : 2010
ISSN
0340-2096
Volume/pages
39:4(2010), p. 376-384
ISI
000280058600006
Full text (Publishers DOI)
Full text (publishers version - intranet only)
UAntwerpen
Faculty/Department
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 11.04.2012
Last edited 18.04.2017