Title
Simple and quick method for whole-liver decellularization : a novel in vitro three-dimensional bioengineering tool? Simple and quick method for whole-liver decellularization : a novel in vitro three-dimensional bioengineering tool?
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Veterinary Sciences
Publication type
article
Publication
Berlin ,
Subject
Pharmacology. Therapy
Veterinary medicine
Source (journal)
Archives of toxicology. - Berlin
Volume/pages
85(2011) :6 , p. 607-612
ISSN
0340-5761
ISI
000291163000008
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Proof of principle of organ reengineering through the development of a transplantable recellularized liver graft was published recently. As the decellularization time of the rat liver took 72 h, loss of some key matrix proteins seemed inevitable. Here, we describe the development of a three-dimensional naturally derived liver scaffold with an intact microvascular system that is capable of withstanding fluid flows in the three hepatic circular systems and that is obtained within 60 min. For this purpose, whole rat livers were sequentially perfused with a selection of mild tensioactive substances to remove the cellular components while preserving the major extracellular matrix proteins, including laminin, collagen I, collagen IV, and fibronectin. In addition, we could show the presence of extracellular matrixbound growth factor islets, important for cell engraftment, migration, proliferation, and differentiation. This easy to prepare scaffold could represent a remarkable tool in the bioengineering of complex three-dimensional in vitro systems for advanced preclinical drug development.
E-info
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