Publication
Title
Stereology and computer assisted three-dimensional reconstruction as tools to study probiotic effects of **Aeromonas hydrophila** on the digestive tract of germ-free **Artemia franciscana** nauplii
Author
Abstract
Aims:  Validation of stereology and three-dimensional reconstruction for monitoring the probiotic effect of Aeromonas hydrophila on the gut development of germ-free Artemia franciscana nauplii. Methods and Results:  Germ-free Artemia nauplii were cultured using Bakers yeast and dead Aer. hydrophila. Live Aer. hydrophila were added on the first day to the treatment group. The gut length and volume were monitored on days two and four using stereology and three-dimensional reconstruction. Both methods showed comparable results. Stereology was least labour intensive to estimate volumes, while three-dimensional reconstructions rendered architectural and topographical data of the gut. Moreover, a positive effect of probiotic bacterium, Aer. hydrophila is likely. Conclusion:  Slight increment in the growth of the digestive tract of A. franciscana nauplii exerted by probiotic bacteria could be detected using stereology and three-dimensional reconstruction. Significance and Impact of the Study:  The gnotobiotic Artemia rearing system is unique to investigate the effects of micro-organisms on the development of nauplii. However, in the base of this model system, only survival counts and length measurements exist as monitoring tools. Therefore, additional tools such as stereology and three-dimensional reconstruction are prerequisite to obtain more powerful analysis.
Language
English
Source (journal)
The journal of applied microbiology. - London
Publication
London : 2011
ISSN
1364-5072
Volume/pages
110:1(2011), p. 98-105
ISI
000285207600011
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 11.04.2012
Last edited 01.07.2017