Title
Proliferation patterns in the canine endometrium during the estrous cycle Proliferation patterns in the canine endometrium during the estrous cycle
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Veterinary Sciences
Publication type
article
Publication
Los Altos, Calif. ,
Subject
Veterinary medicine
Source (journal)
Theriogenology: an international journal of animal reproduction. - Los Altos, Calif.
Volume/pages
62(2004) :3/4 , p. 631-641
ISSN
0093-691X
ISI
000227659100024
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The proliferative activity in the endometrium of 58 bitches in different stages of the estrous cycle was assessed by immunohistochemical detection of the Ki-67 proliferation associated nuclear antigen and by counting mitotic figures. The Ki-67 labelling index and the mitotic index were determined in the surface epithelium, the stroma, the crypts and the basal glands by calculating the percentage of Ki-67 positive cells and mitotic figures, respectively, on a total of 500 cells of each category. Endometrial vascular proliferation was also verified by counting the number of Ki-67 positive cells on a total of 100 endothelial cells. The present study showed two proliferation peaks involving different cell groups. In the surface epithelium, the stroma, the blood vessels and the crypts, the highest labelling and mitotic indexes were noticed during proestrus, whereas for the basal glands these indexes significantly increased (P<0.05) during estrus compared to late metestrus and anestrus. Furthermore, a slightly positive correlation (P<0.05) was found between the labelling index in the basal glands and the serum progesterone levels, whereas the labelling indexes in the other cell groups were positively correlated with the estradiol-17β levels, although not always significantly. These findings suggest that regulation of the proliferation in the surface epithelium, the stroma, the blood vessels and the crypts is different from the proliferation in the basal glands.
E-info
https://repository.uantwerpen.be/docman/iruaauth/98b37a/4bb1e0e513e.pdf
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