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Title
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Biological and chemical approaches for the detection and identification of illegal estrogens in water-based solutions
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Author
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Abstract
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| The continuous introduction of new products used as growth promoters in animal husbandry, for sports doping and as products for body-building requires residue laboratories to initiate research on developing a strategy for the identification of unknown components. In this study, a strategy is presented for elucidating the identity, the structure and the possible effects of illegal estrogenic compounds in an unidentified water-based solution. To obtain complete information on the composition and activity of the unidentified product, a multidisciplinary approach was needed. A case-study is described with a solution X found during a raid. First, in vivo techniques (animal trials with mice, anatomical and histological research) were combined with in vitro techniques (the yeast estrogenic screen (YES)). In a later stage of the investigation, HPLC-fractionation, liquid chromatographymultiple mass spectrometry (LC-MS n and gas chromatography-multiple mass spectrometry (GC-MS n were used. Finally, the identity of solution X was confirmed in a very low concentration range (10 ng/L estrone and 400 ng/l ethinyloestradiol). |
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Language
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English
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Source (journal)
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Veterinary research communications. - Dordrecht, 1980, currens
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Publication
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Dordrecht
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Kluwer Academic Publishers
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2006
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ISSN
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0165-7380
[print]
1573-7446
[online]
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DOI
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10.1007/S11259-006-3326-0
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Volume/pages
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30
:6
(2006)
, p. 577-585
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ISI
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000239023500001
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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