Title
Biological and chemical approaches for the detection and identification of illegal estrogens in water-based solutions
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Veterinary Sciences
Publication type
article
Publication
Amsterdam ,
Subject
Veterinary medicine
Source (journal)
Veterinary research communications. - Amsterdam
Volume/pages
30(2006) :6 , p. 577-585
ISSN
0165-7380
ISI
000239023500001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
The continuous introduction of new products used as growth promoters in animal husbandry, for sports doping and as products for body-building requires residue laboratories to initiate research on developing a strategy for the identification of unknown components. In this study, a strategy is presented for elucidating the identity, the structure and the possible effects of illegal estrogenic compounds in an unidentified water-based solution. To obtain complete information on the composition and activity of the unidentified product, a multidisciplinary approach was needed. A case-study is described with a solution X found during a raid. First, in vivo techniques (animal trials with mice, anatomical and histological research) were combined with in vitro techniques (the yeast estrogenic screen (YES)). In a later stage of the investigation, HPLC-fractionation, liquid chromatographymultiple mass spectrometry (LC-MS n and gas chromatography-multiple mass spectrometry (GC-MS n were used. Finally, the identity of solution X was confirmed in a very low concentration range (10 ng/L estrone and 400 ng/l ethinyloestradiol).
E-info
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