Title
|
|
|
|
Biological and chemical approaches for the detection and identification of illegal estrogens in water-based solutions
|
|
Author
|
|
|
|
|
|
Abstract
|
|
|
|
The continuous introduction of new products used as growth promoters in animal husbandry, for sports doping and as products for body-building requires residue laboratories to initiate research on developing a strategy for the identification of unknown components. In this study, a strategy is presented for elucidating the identity, the structure and the possible effects of illegal estrogenic compounds in an unidentified water-based solution. To obtain complete information on the composition and activity of the unidentified product, a multidisciplinary approach was needed. A case-study is described with a solution X found during a raid. First, in vivo techniques (animal trials with mice, anatomical and histological research) were combined with in vitro techniques (the yeast estrogenic screen (YES)). In a later stage of the investigation, HPLC-fractionation, liquid chromatographymultiple mass spectrometry (LC-MS n and gas chromatography-multiple mass spectrometry (GC-MS n were used. Finally, the identity of solution X was confirmed in a very low concentration range (10 ng/L estrone and 400 ng/l ethinyloestradiol). |
|
|
Language
|
|
|
|
English
|
|
Source (journal)
|
|
|
|
Veterinary research communications. - Dordrecht, 1980, currens
|
|
Publication
|
|
|
|
Dordrecht
:
Kluwer Academic Publishers
,
2006
|
|
ISSN
|
|
|
|
0165-7380
[print]
1573-7446
[online]
|
|
DOI
|
|
|
|
10.1007/S11259-006-3326-0
|
|
Volume/pages
|
|
|
|
30
:6
(2006)
, p. 577-585
|
|
ISI
|
|
|
|
000239023500001
|
|
Full text (Publisher's DOI)
|
|
|
|
|
|
Full text (publisher's version - intranet only)
|
|
|
|
|
|