Title
Ability of **Cricetomys** rats to detect **Mycobacterium tuberculosis** and discriminate it from other microorganisms Ability of **Cricetomys** rats to detect **Mycobacterium tuberculosis** and discriminate it from other microorganisms
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Edinburgh ,
Subject
Biology
Human medicine
Source (journal)
Tuberculosis / International Union against Tuberculosis and Lung Disease. - Edinburgh, 2001, currens
Volume/pages
92(2012) :2 , p. 182-186
ISSN
1472-9792
ISI
000300619800009
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Trained African giant pouched rats (Cricetomys gambianus) have potential for diagnosis of tuberculosis (TB). These rats target volatile compounds of Mycobacterium tuberculosis (Mtb) that cause TB. Mtb and nontuberculous mycobacteria (NTM) species are related to Nocardia and Rhodococcus spp., which are also acid-fast bacilli and can be misdiagnosed as Mtb in smear microscopy. Diagnostic performance of C. gambianus on in vitro-cultured mycobacterial and related pulmonary microbes is unknown. This study reports on the response of TB detection rats to cultures of reference Mtb, clinical Mtb, NTM, Nocardia; Rhodococcus; Streptomyces; Bacillus; and yeasts. Trained rats significantly discriminated Mtb from other microbes (p < 0.008, Fisher's exact test). Detection of Mtb cultures was age-related, with exponential and early stationary phase detected more frequently than early log phase and late stationary phase (p < 0.001, Fisher's test) (sensitivity -83.33%, specificity -94.4%, accuracy -94%). The detection of naturally TB-infected sputum exceeded that of negative sputum mixed with Mtb, indicating that C. gambianus are conditioned to detect odours of TB-positive sputum better than spiked sputum. Although further studies on volatiles from detectable growth phases of Mtb are vital for identification of Mtb-specific volatiles detected by rats, our study underline the potential of C. gambianus for TB diagnosis. (C) 2011 Elsevier Ltd. All rights reserved.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/b2d00c/1483.pdf
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