Title
Validation of the MicroScan-96 for the species identification and methicillin susceptibility testing of clinical significant coagulase-negative staphylococci Validation of the MicroScan-96 for the species identification and methicillin susceptibility testing of clinical significant coagulase-negative staphylococci
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Wiesbaden ,
Subject
Biology
Human medicine
Source (journal)
European journal of clinical microbiology and infectious diseases. - Wiesbaden
Volume/pages
31(2012) :5 , p. 747-751
ISSN
0934-9723
ISI
000302481600015
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
An automated system, MicroScan WalkAway-96, in conjugation with Combo PosA (R) 28 panels, was validated for the identification and methicillin susceptibility of coagulase-negative staphylococci (CNS). The performance of this system was evaluated on 428 CNS. Identification results were compared using a validated in-house method. Methicillin susceptibility was compared with oxacillin MIC testing and the presence of the mecA gene by PCR (in-house real-time method). The MicroScan system correctly identified 94.6% of the staphylococci (405 out of 428). 3.5% of the strains (15 out of 428) were not correctly identified. 1.9% of the isolates (8 out of 428) were correctly identified with a low probability. Identification of Staphylococcus warneri and Staphylococcus lugdunensis was determined with the least accuracy. Microscan combines both oxacillin and cefoxitin for determination of the methicillin susceptibility result. Correlation between this result and the mecA method was 97.6%. Correlation with the oxacillin MIC method was also 97.6%. Fourteen isolates showed a discrepant result, 8 were reported to be resistant in mecA-negative strains, 2 were reported false-susceptible in mecA positive strains and 4 strains showed a discrepant result with oxacillin MIC, but not with mecA determination. The automated system can be considered a simple and reliable method for identification and methicillin susceptibility of CNS.
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