Title
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Effect of serine/threonine protein kinases and protein phosphatases inhibitors on mitosis progression in a synchronized tobacco BY-2 culture
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Author
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Abstract
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In order to investigate the role of various serine/threonine protein kinases and protein phosphatases in the regulation of mitosis progression in plant cells, the influence of cyclin(olomoucine) and Ca2+/calmodulin-dependent (W7) protein kinases inhibitors, as well as protein kinase C inhibitors (H7 and staurosporine), and a protein phosphatases inhibitor (okadaic acid) on mitosis progression in synchronized tobacco BY-2 cells has been studied. It was found that BY-2 culture treatment with inhibitors of cyclin-dependent protein kinases and protein kinase C caused a prophase delay, reduced the mitotic index, and displaced the mitotic peak as compared with control cells. Inhibition of Ca2+/calmodulin-dependent protein kinases enhanced the cells entry into prophase and delayed their exit from mitosis. Meanwhile inhibition of serine/threonine protein phosphatases insignificantly enhances synchronized BY-2 cells entering into all phases of mitosis. |
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Language
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English
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Source (journal)
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Cytology and genetics / Polska Akademia Nauk. Komitet Nauk Chemicznych. - New York
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Publication
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New York
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2012
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ISSN
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0095-4527
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DOI
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10.3103/S009545271202003X
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Volume/pages
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46
:2
(2012)
, p. 89-95
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ISI
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000302957200003
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Full text (Publisher's DOI)
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Full text (publisher's version - intranet only)
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