Title
qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
London ,
Subject
Human medicine
Source (journal)
Genome biology. - London
Volume/pages
8(2007) :2 , p. R19,1-R19,14
ISSN
1465-6906
ISI
000246076300017
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data.
Full text (open access)
https://repository.uantwerpen.be/docman/irua/c8de68/713a1cdc.pdf
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