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Title
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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data
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Author
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Abstract
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| Although quantitative PCR (qPCR) is becoming the method of choice for expression profiling of selected genes, accurate and straightforward processing of the raw measurements remains a major hurdle. Here we outline advanced and universally applicable models for relative quantification and inter-run calibration with proper error propagation along the entire calculation track. These models and algorithms are implemented in qBase, a free program for the management and automated analysis of qPCR data. |
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Language
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English
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Source (journal)
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Genome biology. - London
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Publication
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London
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2007
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ISSN
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1465-6906
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DOI
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10.1186/GB-2007-8-2-R19
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Volume/pages
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8
:2
(2007)
, p. R19,1-R19,14
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ISI
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000246076300017
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Full text (Publisher's DOI)
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Full text (open access)
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