Site-specific labeling of second generation annexin V with <script type="math/tex">^{99m}Tc(CO)_{3}</script> for improved imaging of apoptosis in vivo

De Saint-Hubert, Marijke; Mottaghy, Felix M.; Vunckx, Kathleen; Stroobants, Sigrid; et al.

doi:10.1016/J.BMC.2009.12.021

Title

Site-specific labeling of second generation annexin V with $^{99m}Tc(CO)_{3}$ for improved imaging of apoptosis in vivo

Author

De Saint-Hubert, Marijke

Mottaghy, Felix M.

Vunckx, Kathleen

Stroobants, Sigrid

et al.

Abstract

In this study second generation AnxV was specifically labeled with 99mTc in three different ways outside the binding region of the protein to obtain an improved target-to-background activity ratio. The compounds were tested in vitro and in vivo in normal mice and in a model of hepatic apoptosis (anti-Fas mAb). The apoptosis binding was most prominent for the HIS-tagged second generation AnxV labeled with 99mTc(CO)3 in comparison to 99mTc-HYNIC-cys-AnxV and 99mTc(CO)3-DTPA-cys-AnxV.

Language

English

Source (journal)

Bioorganic and medicinal chemistry. - Oxford

Publication

Oxford : 2010

ISSN

0968-0896

DOI

10.1016/J.BMC.2009.12.021

Volume/pages

18 :3 (2010) , p. 1356-1363