Title
Site-specific labeling of second generation annexin V with <tex>$^{99m}Tc(CO)_{3}$</tex> for improved imaging of apoptosis in vivo Site-specific labeling of second generation annexin V with <tex>$^{99m}Tc(CO)_{3}$</tex> for improved imaging of apoptosis in vivo
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Oxford ,
Subject
Biology
Human medicine
Source (journal)
Bioorganic and medicinal chemistry. - Oxford
Volume/pages
18(2010) :3 , p. 1356-1363
ISSN
0968-0896
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Abstract
In this study second generation AnxV was specifically labeled with 99mTc in three different ways outside the binding region of the protein to obtain an improved target-to-background activity ratio. The compounds were tested in vitro and in vivo in normal mice and in a model of hepatic apoptosis (anti-Fas mAb). The apoptosis binding was most prominent for the HIS-tagged second generation AnxV labeled with 99mTc(CO)3 in comparison to 99mTc-HYNIC-cys-AnxV and 99mTc(CO)3-DTPA-cys-AnxV.
E-info
https://repository.uantwerpen.be/docman/iruaauth/c656f7/f0be43b073d.pdf