Title
Establishment of a mouse gastrointestinal stromal tumour model and evaluation of response to imatinib by small animal positron emission tomography Establishment of a mouse gastrointestinal stromal tumour model and evaluation of response to imatinib by small animal positron emission tomography
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Athens ,
Subject
Human medicine
Source (journal)
Anticancer research. - Athens
Volume/pages
26(2006) :2:A , p. 1247-1252
ISSN
0250-7005
ISI
000236674800058
Carrier
E
Target language
English (eng)
Abstract
Background: Gastrointestinal stromal tumours (GIST) predominantly express activating mutations of the KIT tyrosine kinase receptor and are successfully treated with imatinib mesylate, a KIT inhibitor. As resistance to imatinib causes therapy failure, our aim was to develop an in vivo GIST model to evaluate KIT inhibitors and monitor therapy with small animal positron emission tomography (PET). Materials and Methods: The first mouse model of GIST xenografts was successfully established by injecting GIST882 cells subcutaneously into nude mice. Results: Using the small animal PET, FDG up-take in xenografts was significantly decreased after 24 h of treatment with imatinib, which correlated with a response to treatment, e.g., with a decrease in tumour volume, the inhibition of KIT and downstream intermediate phosphorylation and arrest of tumour cell proliferation as evaluated after 7 days of treatment. Conclusion: This model is useful to study imatinib resistance and to evaluate novel targeted therapies.
E-info
https://repository.uantwerpen.be/docman/iruaauth/832f37/a1310a03ad0.pdf
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