Title
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Aptasensing of chloramphenicol in the presence of its analogues : reaching the maximum residue limit
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Author
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Abstract
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A novel label-free folding induced aptamer-based electrochemical biosensor for the detection of chloramphenicol (CAP) in the presence of its analogues has been developed. CAP is a broad-spectrum antibiotic which has lost its favor due to its serious adverse toxic effects on human health. Aptamers are artificial nucleic acid ligands (ssDNA or RNA) able to specifically recognize a target such as CAP. In this article, the aptamers are fixed onto a gold electrode surface by a self-assembly approach. In the presence of CAP, the unfolded ssDNA on the electrode surface changes to a hairpin structure bringing the target molecules close to the surface and trigger electron transfer. Detection limits were determined to be 1.6×10-9 mol L-1. In addition, thiamphenicol (TAP) and florfenicol (FF), antibiotics with a similar structure to CAP, did not influence the performance of the aptasensor, suggesting a good selectivity of the CAP-aptasensor. Simplicity and lower detection limit (because of the home-selected aptamers) make that the electrochemical aptasensor is suitable for practical use in the detection of CAP in milk samples. |
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Language
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English
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Source (journal)
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Analytical chemistry. - Washington, D.C., 1948, currens
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Publication
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Washington, D.C.
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2012
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ISSN
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0003-2700
[print]
5206-882X
[online]
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DOI
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10.1021/AC3012522
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Volume/pages
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84
:15
(2012)
, p. 6753-6758
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ISI
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000307159200069
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Full text (Publisher's DOI)
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