Title
Interferon-<tex>$\gamma$</tex> modulates the functional profile of in-vitro-cultured porcine microglia
Author
Faculty/Department
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Veterinary Sciences
Faculty of Medicine and Health Sciences
Publication type
article
Publication
Oxford ,
Subject
Biology
Human medicine
Source (journal)
Neuroreport. - Oxford
Volume/pages
23(2012) :9 , p. 519-524
ISSN
0959-4965
ISI
000304317200001
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Microglia are the most important immune cells within the highly specialized environment of the central nervous system. Upon activation, they transform from a resting 'ramified' into a fully functional 'amoeboid' phenotype with the ability to perform phagocytosis and generate free radicals. A combined flow cytometric assay for the simultaneous measurement of these two functions in porcine microglia in vitro is presented: reactive oxygen species are detected using hydroethidine; phagocytosis is assessed using fluorescein isothiocyanate-labeled latex beads. The combination of these two probes allowed us to distinguish four subpopulations within cultured porcine microglia on the basis of their functional activity. The effect of several exogenous stimuli [phorbol myristate acetate, conditioned medium, interferon gamma (IFN-gamma)] on the in-vitro functional properties of porcine microglia is investigated using this test. In particular for IFN-gamma, a significant modulatory effect on the intracellular reactive oxygen species production and phagocytic activity was observed. This result suggests an alternative role of IFN-gamma acting on cultured porcine microglia. NeuroReport 23:519-524 (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins.
E-info
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