Publication
Title
Retinoic acid potentiates TNF-alpha-induced ICAM-1 expression in normal human epidermal keratinocytes
Author
Abstract
ICAM-1 protein in keratinocytes is thought to contribute to cutaneous inflammatory reactions. Its induction depends-among others-on cytokines such as TNF-alpha, IFN-gamma, IL-1 or on retinoic acid (RA), a key regulator of epidermal homeostasis. We investigated the effect of treatments with TNF-alpha, RA or their combination on ICAM-1 expression on proliferative or differentiating keratinocytes over an 8 day culture period. Basal ICAM-1 levels were undetectable at low (30 mu M) and standard (88 mu M) Ca2+ and RA alone did not induce ICAM-1. However, at high Ca2+ (1500 mu M), ICAM-1 levels were augmented in response to RA-treatment. TNF-alpha induced a transient ICAM-1 increase in NHK, which reached peak-levels 2-4 days post cytokine stimulus. RA. potentiated the TNF-alpha-induced ICAM-1 response in all Ca2+-concentrations. This potentiating effect of RA was confirmed at the mRNA level. In summary, our results establish retinoic acid as an enhancer of TNF-alpha-induced ICAM-1 levels in NHK, (C) 1999 Academic Press.
Language
English
Source (journal)
Biochemical and biophysical research communications. - New York, N.Y.
Publication
New York, N.Y. : 1999
ISSN
0006-291X
Volume/pages
255:1(1999), p. 64-69
ISI
000078599700012
Full text (Publisher's DOI)
UAntwerpen
Publication type
Subject
External links
Web of Science
Record
Identification
Creation 12.07.2012
Last edited 13.11.2017