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Title
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Retinoic acid potentiates TNF-alpha-induced ICAM-1 expression in normal human epidermal keratinocytes
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Author
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Abstract
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| ICAM-1 protein in keratinocytes is thought to contribute to cutaneous inflammatory reactions. Its induction depends-among others-on cytokines such as TNF-alpha, IFN-gamma, IL-1 or on retinoic acid (RA), a key regulator of epidermal homeostasis. We investigated the effect of treatments with TNF-alpha, RA or their combination on ICAM-1 expression on proliferative or differentiating keratinocytes over an 8 day culture period. Basal ICAM-1 levels were undetectable at low (30 mu M) and standard (88 mu M) Ca2+ and RA alone did not induce ICAM-1. However, at high Ca2+ (1500 mu M), ICAM-1 levels were augmented in response to RA-treatment. TNF-alpha induced a transient ICAM-1 increase in NHK, which reached peak-levels 2-4 days post cytokine stimulus. RA. potentiated the TNF-alpha-induced ICAM-1 response in all Ca2+-concentrations. This potentiating effect of RA was confirmed at the mRNA level. In summary, our results establish retinoic acid as an enhancer of TNF-alpha-induced ICAM-1 levels in NHK, (C) 1999 Academic Press. |
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Language
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English
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Source (journal)
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Biochemical and biophysical research communications. - New York, N.Y.
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Publication
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New York, N.Y.
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1999
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ISSN
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0006-291X
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DOI
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10.1006/BBRC.1999.0147
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Volume/pages
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255
:1
(1999)
, p. 64-69
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ISI
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000078599700012
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Full text (Publisher's DOI)
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