Characterization of <tex>$\omega$</tex>-3-docosahexaenoic acid-containing molecular species of phospholipids in rainbow trout liver
Faculty of Pharmaceutical, Biomedical and Veterinary Sciences. Pharmacy
Journal of agricultural and food chemistry. - Washington, D.C.
, p. 3120-3125
University of Antwerp
Four major phospholipids from rainbow trout liver were purified by chromatography, and the phospholipid classes and their molecular species were quantitatively and qualitatively determined by P-31 nuclear magnetic resonance, gas chromatography, liquid secondary ion mass spectrometry, and liquid secondary ion mass spectrometry combined with tandem mass spectrometry. Trout liver phospholipids consist mainly of glycerophosphatidylcholine (GPC; 67%), glycerophosphatidylethanolamine (GPE; 22%), glycerophosphatidylserine (GPS; 10%), and glycerophosphatidylinositol (GPI; 1.6%); the contents of 22:6 omega-3 fatty acid in the first three phospholipid classes were 34% (GPC), 31% (GPE), and 27% (GPS), respectively, and abundant molecular species were 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (GPC 16:0-22:6) (see Table 2 for phospholipid molecular species designations), 1-eicosaenoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 20: 1-22:6), 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 18:0-22:6), 1-oleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 18:1-22:6), 1-linoleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 18:2-22:6), 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 16:0-22:6), 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoserine (GPS 16:0-22:6), and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoserine (GPS 18:0-22:6). GPI in trout liver lacks molecular species containing 22:6 omega-3 fatty acid. The study clearly indicates that a very high content of 22:6 omega-3 fatty acid-containing molecular species within phospholipids, mainly within GPC and GPE, is characteristic of rainbow trout liver.