Publication
Title
Detection of **Mycoplasma pneumoniae** by two polymerase chain reactions and role of M-pneumoniae in acute respiratory tract infections in pediatric patients
Author
Abstract
Mycoplasma pneumoniae and viruses in acute respiratory tract infections in children were studied during the winter of 1992-1993 in Antwerp, Belgium, M. pneumoniae was diagnosed in nasopharyngeal aspirates by culture and polymerase chain reaction (PCR). For this, amplification of a fragment of the P1 adhesin gene in samples prepared by two methods was compared in two laboratories, and in one laboratory, a fragment of the 16S rRNA gene was amplified. The sensitivity of culture versus PCR was 61.5%, Provided a specific internal control is used, sample preparation by freeze-boiling combined with PCR for the P1 gene and amplicon detection by visual inspection of the electrophoresis gel can be recommended, although maximal results are obtained after hybridization, M. pneumoniae was present in 0.5% of patients <2 years old and 6.9% of patients >2. M. pneumoniae was second to respiratory syncytial virus or detected equally in lower respiratory infections.
Language
English
Source (journal)
The journal of infectious diseases. - Chicago, Ill.
Publication
Chicago, Ill. : 1996
ISSN
0022-1899
Volume/pages
173:6(1996), p. 1445-1452
ISI
A1996UM11500019
Full text (Publisher's DOI)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 19.07.2012
Last edited 19.10.2017
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