Publication
Title
Partial-purification and further characterization of the novel endoglucosaminidase from human serum that hydrolyzes 4-methylumbelliferyl-N-acetyl--D-chitotetraoside (MU-TACT hydrolase)
Author
Abstract
A novel endoglucosaminidase, originally described by Den Tandt et al. [Int. J. Biochem. 20 (1988), 713-719] and bearing the provisional name MU-TACT hydrolase, was purified from human serum 56,000-fold by means of ammonium sulphate precipitation, anion-exchange chromatography, Con A-Sepharose chromatography and gel filtration on Sepharose CL-6B followed by Superose 12 HR. Based on the latter technique the native apparent molecular weight of the enzyme appeared to be equal to that of myoglobin, being approx. 17 kD. The enzyme eluted clearly at a different volume than lysozyme. MU-TACT is a commercially available substrate for lysozyme. For unknown reasons two major peptides co-purify that give bands on SDS-PAGE of 55-60 and 31 kD, respectively.
Language
English
Source (journal)
The international journal of biochemistry. - Oxford, 1970 - 1994
Publication
Oxford : 1994
ISSN
0020-711X
Volume/pages
26:12(1994), p. 1369-1375
ISI
A1994QD19700006
Full text (Publisher's DOI)
Full text (publisher's version - intranet only)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 19.07.2012
Last edited 07.08.2017
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