Title
Epitope mapping of monoclonal antibodies to the paired helical filaments of Alzheimer's disease : identification of phosphorylation sites in tau-protein Epitope mapping of monoclonal antibodies to the paired helical filaments of Alzheimer's disease : identification of phosphorylation sites in tau-protein
Author
Faculty/Department
Faculty of Medicine and Health Sciences
Publication type
article
Publication
London ,
Subject
Chemistry
Biology
Source (journal)
The biochemical journal. - London, 1984, currens
Volume/pages
301(1994) :Part 3 , p. 871-877
ISSN
0264-6021
1470-8728
ISI
A1994PB31100033
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
Tau is a neuronal phosphoprotein the expression of which is developmentally regulated. A single tau isoform is expressed in fetal human brain but six isoforms are expressed in adult human brain, with the fetal isoform corresponding to the shortest adult isoform. Phosphorylation is also developmentally regulated, as fetal tau is phosphorylated at more sites than adult tau. In Alzheimer's disease, the six adult tau isoforms become hyperphosphorylated and form the paired helical filament (PHF), the major fibrous component of the neurofibrillary lesions. One way to identify phosphorylated sites in tau is to use antibodies that recognize phosphorylated residues within a specific amino acid sequence. We here characterize the two novel phosphorylation-dependent anti-tau antibodies AT270 and AT180 and identify their epitopes as containing phosphorylated Thr-181 and Thr-231 respectively. With these antibodies we show that these two threonine residues are partially phosphorylated in fetal and adult tau and almost fully phosphorylated in PHF tau. This result contrasts with previous studies of Ser-202 and Ser-396 which are partially phosphorylated in fetal tan, unphosphorylated in adult tau but almost fully phosphorylated in PHF tau.
E-info
https://repository.uantwerpen.be/docman/iruaauth/2bf19a/5e44371.pdf
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