Publication
Title
Light-scattering by bovine alpha-crystallin proteins in solution : hydrodynamic structure and interparticle interaction
Author
Abstract
We have studied diluted bovine eye lens alpha-crystallin solutions by using light scattering. The protein particles were modeled as hard spheres, showing electrostatic repulsion, due to surplus electric charges, and weak attractive interaction. The repulsive potential V-R is defined by the radius of the particles, the Debye length K-1, and the number of charges at the Gouy layer; the attractive potential has been described by the London-van der Waals potential and is defined by the Hamaker constant A. We have used the diluted gas approximation and the one component macrofluid model to relate the experimental static factor K-1 to the theoretical expression of the interaction potential V(x). This resulted in a Hamaker constant A of 0.06 +/- 0.01 KBT and an effective charge q ranging from 18 +/- 1 at low ionic strength (Omega = 0.0022 M) to 50 +/- 5 at high ionic strength(Omega = 0.1472M).
Language
English
Source (journal)
Biophysical journal. - New York, N.Y.
Publication
New York, N.Y. : 1994
ISSN
0006-3495
Volume/pages
66:3 Part 1(1994), p. 861-872
ISI
A1994MZ35300033
Full text (Publisher's DOI)
Full text (open access)
UAntwerpen
Faculty/Department
Research group
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Web of Science
Record
Identification
Creation 19.07.2012
Last edited 14.10.2017
To cite this reference