Title
Effect of oxygen and salt on haemolymph oxygen binding in the brine shrimp Artemia franciscana
Author
Faculty/Department
Faculty of Sciences. Biology
Publication type
article
Publication
Berlin ,
Subject
Biology
Source (journal)
Marine biology
Marine biology. - Berlin
Volume/pages
118(1994) :2 , p. 263-269
ISSN
0025-3162
ISI
A1994MY34200011
Carrier
E
Target language
English (eng)
Full text (Publishers DOI)
Affiliation
University of Antwerp
Abstract
The oxygen-binding properties of haemolymph from laboratory-reared Artemia franciscana were investigated in vitro. Adult female brine shrimp without eggs were acclimated to seven different combinations of salinity and oxygen. The oxygen affinity (P-50) of unprocessed haemolymph rises with acclimation oxygen partial pressure (PO2) up to normoxic values, after which no influence of oxygen occurs. The increase in P-50 with acclimation PO2 can be explained by the change in proportion of each of three different haemoglobins in the haemolymph. Salinity acclimation has no effect on haemolymph P-50. The effects of the different major salts [NaNO3, NaCl, Ca(NO3)(2), Mg(NO3)(2)], pH, and the metal-binding ligand EGTA on the oxygen-binding properties of buffered haemolymph (of shrimp acclimated to 10 parts per thousand salinity) were also studied. Little or no effect of these salts could be found. A small Bohr effect (pH 6.5 to 9.1, phi = - 0.11) was noted. Addition of EGTA caused a significant decrease of the oxygen affinity at concentrations up to 50 mmoll(-l).
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