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Title
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Effect of oxygen and salt on haemolymph oxygen binding in the brine shrimp Artemia franciscana
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Author
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Abstract
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| The oxygen-binding properties of haemolymph from laboratory-reared Artemia franciscana were investigated in vitro. Adult female brine shrimp without eggs were acclimated to seven different combinations of salinity and oxygen. The oxygen affinity (P-50) of unprocessed haemolymph rises with acclimation oxygen partial pressure (PO2) up to normoxic values, after which no influence of oxygen occurs. The increase in P-50 with acclimation PO2 can be explained by the change in proportion of each of three different haemoglobins in the haemolymph. Salinity acclimation has no effect on haemolymph P-50. The effects of the different major salts [NaNO3, NaCl, Ca(NO3)(2), Mg(NO3)(2)], pH, and the metal-binding ligand EGTA on the oxygen-binding properties of buffered haemolymph (of shrimp acclimated to 10 parts per thousand salinity) were also studied. Little or no effect of these salts could be found. A small Bohr effect (pH 6.5 to 9.1, phi = - 0.11) was noted. Addition of EGTA caused a significant decrease of the oxygen affinity at concentrations up to 50 mmoll(-l). |
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Language
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English
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Source (journal)
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Marine biology. - Berlin, 1967, currens
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Marine biology
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Publication
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Berlin
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1994
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ISSN
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0025-3162
[print]
1432-1793
[online]
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DOI
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10.1007/BF00349793
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Volume/pages
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118
:2
(1994)
, p. 263-269
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ISI
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A1994MY34200011
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Full text (Publisher's DOI)
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