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Title
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Oxidative stress in donor mares for ovum pick-up delays embryonic development
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Author
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Abstract
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| The in vitro production of equine embryos via ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) has increased rapidly. There is a marked effect of the individual mare on the outcome of OPU-ICSI, but little is known about the influence of the mare's health condition. This study aimed to investigate the potential associations between the concentrations of interleukin-6 (IL-6), reactive oxygen metabolites (d-ROMs), and biological antioxidant potential (BAP) in serum of oocytes' donor mares and the subsequent embryonic development. Just before OPU, a blood sample was collected from 28 Warmblood donor mares, that were subjected to a routine OPU-ICSI program. The serum concentrations of IL-6, d-ROMs, and BAP were assayed photometrically. The maturation, cleavage and blastocyst rate as well as the kinetics of blastocyst development were recorded. The average blastocyst rate was 24.68 +/- 5.16% and the average concentrations of IL-6, d-ROMs, and BAP were 519.59 +/- 157.08 pg/mL, 171.30 +/- 4.55 carratelli units (UCARR), and 2711.30 +/- 4.55 mu mol/L, respectively. Serum concentrations of IL-6, d-ROMs, and BAP were not significantly different between mares yielding at least one blastocyst (552.68 +/- 235.18 pg/mL, 168.36 +/- 5.56 UCARR, and 2524.80 +/- 159.55 mu mol/L) and mares yielding no blastocysts (468.47 +/- 179.99 pg/mL, 175.85 +/- 7.89 UCARR, and 2999.50 +/- 300.13 mu mol/L, respectively). Serum concentrations of d-ROMs were significantly lower in mares with fast growing (at day 7-8 post ICSI; 148.10 +/- 8.13 UCARR) compared to those with slow growing blastocysts (>= day 9 post ICSI; 179.41 +/- 4.89 UCARR; P = 0.003). Taken together, the serum concentration of IL-6, d-ROMs, and BAP do not determine the mare's ability to produce blastocysts in vitro. Although it may be questioned whether a single sample is representative of the mare's health status, changes in serum metabolites related to oxidative stress at the time of oocyte retrieval were linked to a delayed blastocyst development in a clinical OPU-ICSI outcome. |
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Language
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English
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Source (journal)
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Theriogenology: an international journal of animal reproduction. - Los Altos, Calif.
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Publication
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Los Altos, Calif.
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2024
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ISSN
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0093-691X
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DOI
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10.1016/J.THERIOGENOLOGY.2023.10.006
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Volume/pages
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213
(2024)
, p. 109-113
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ISI
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001100120900001
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Pubmed ID
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37826984
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Full text (Publisher's DOI)
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Full text (open access)
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The author-created version that incorporates referee comments and is the accepted for publication version Available from 10.10.2024
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Full text (publisher's version - intranet only)
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