Publication
Title
Non-viral delivery of RNA for therapeutic T cell engineering
Author
Abstract
Adoptive T cell transfer has shown great success in treating blood cancers, resulting in a growing number of FDA-approved therapies using chimeric antigen receptor (CAR)-engineered T cells. However, the effectiveness of this treatment for solid tumors is still not satisfactory, emphasizing the need for improved T cell engineering strategies and combination approaches. Currently, CAR T cells are mainly manufactured using gammaretroviral and lentiviral vectors due to their high transduction efficiency. However, there are concerns about their safety, the high cost of producing them in compliance with current Good Manufacturing Practices (cGMP), regulatory obstacles, and limited cargo capacity, which limit the broader use of engineered T cell therapies. To overcome these limitations, researchers have explored non-viral approaches, such as membrane permeabilization and carrier-mediated methods, as more versatile and sustainable alternatives for next-generation T cell engineering. Non-viral delivery methods can be designed to transport a wide range of molecules, including RNA, which allows for more controlled and safe modulation of T cell phenotype and function. In this review, we provide an overview of non-viral RNA delivery in adoptive T cell therapy. We first define the different types of RNA therapeutics, highlighting recent advancements in manufacturing for their therapeutic use. We then discuss the challenges associated with achieving effective RNA delivery in T cells. Next, we provide an overview of current and emerging technologies for delivering RNA into T cells. Finally, we discuss ongoing preclinical and clinical studies involving RNA-modified T cells.
Language
English
Source (journal)
Advanced drug delivery reviews. - Amsterdam
Publication
Amsterdam : 2024
ISSN
0169-409X
DOI
10.1016/J.ADDR.2024.115215
Volume/pages
(2024) , 73 p.
Article Reference
115215
Pubmed ID
38401848
Full text (Publisher's DOI)
Full text (open access)
The author-created version that incorporates referee comments and is the accepted for publication version Available from 23.08.2024
UAntwerpen
Faculty/Department
Research group
Project info
Unravelling the cellular response to photoporation.
Interactive and intelligent cellomics platform.
Nuclear envelope stress in laminopathy patient-derived cardiomyocytes (NStrC).
IMARK. Network for image-based biomarker discovery and evaluation
Publication type
Subject
Affiliation
Publications with a UAntwerp address
External links
Record
Identifier
Creation 25.02.2024
Last edited 28.02.2024
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